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'Mike' Michael J. Moser moser at U.WASHINGTON.EDU
Tue Oct 21 15:43:09 EST 1997


Dear Gordon

I isolated a fortuitous tandem copy of a GFP fusion to the N-terminus
yeast calmodulin.  In this case even over-expression of the double GFP
fusion failed to complement when an integrated fusion containing single
copy GFP did.  The signal I got from one copy of S65T was more than
sufficient to visualize the fusion by eye through the microscope. Just my
2c.

Happy GFPing,

Mike Moser                                            Tel: 206-543-6585
UW Department of Pathology                            FAX: 206-543-3967
Box 357705                                       moser at u.washington.edu
Seattle, WA  98195                 http://weber.u.washington.edu/~moser

On 21 Oct 1997, Gordon Barker wrote:

> Dear Netters
> 
> I'm trying to maximise GFP-signal in my yeast cells to detect relatively
> low levels of protein expressed from a weak promoter and consequently
> have a few quick questions.  I've read on this newsgroup that people
> have successfully fused 2 in-frame copies of GFP to produce an even
> brighter reporter molecule... 




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