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Expression of GFP in filamentous fungi

bottin bottin at cict.fr
Tue Oct 21 10:45:10 EST 1997

A mutant version of GFP has been used successfully in Ustilago maydis 
(Spellig et al. (1996) Mol Gen Genet 252: 503-509). It can be obtained 
from Jen Sheen, e-mail : sheen at frodo.mgh.harvard.edu. Codon usage can be 
a problem; this clone worked well in U. maydis although it had not been 
engineered for this fungus, but for Maize. You need a good promoter as 

Dr Arnaud Bottin
Universite Paul Sabatier
118, route de Narbonne
F-31062 Toulouse cedex 04

Phone:  +33/(0)56 155 67 60
FAX:    +33/(0)56 155 83 78
Courel:  bottin at cict.fr

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