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EGFP vs. emerald

Christopher Logg logg at HSC.USC.EDU
Tue Dec 15 19:56:37 EST 1998


Hi.  If anyone's interested, I've compared the relative brightnesses of
EGFP (Clontech) and emerald GFP (Packard).  In three different
retroviral vector constructs, the emerald gives a notably brighter
signal, both by flow cytometry and by fluorescence microscopy.  The EGFP
and emerald vectors I used are identical save for the 4 point mutations
that distinguish the two GFPs.  So, if anyone's having trouble detecting
their GFP, using emerald might help.

Chris

logg at hsc.usc.edu




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