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Fixer

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Fri Jul 17 08:03:43 EST 1998


CHK <chk108 at PSU.EDU> wrote:
> I am trying to find a way to fix a cell that has been bombarded with a
> GFP/MAP4 construct that is fluorescing for immunolocalization.  The fixer
> has to sustain the fluorescent signal for approx. 12 hrs or more. If you
> know any, please let me know.

I have been rather successful with 4% Paraformaldehyde in PBS. If kept
in the fridge, the GFP-transfected cells still show nice fluorescence
the next morning. (I didn't check after longer time intervals.)

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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