In collaboration with Trends in Cell Biology, Andrew Matus and I are
putting together a CD-ROM dedicated to applications of GFP in the
visualization of dynamic biological processes. The CD-ROM will feature
examples from all areas of biological research and will be distributed as
a freebie. This is an opportunity for you to show your movies and
time-lapse sequences to a broad audience.
So if you would like to have your data considered for inclusion, please
send a message with a brief description of the materials you are able to
provide to gfp at fmi.ch as soon as possible. (You are also welcome to send
your completed contribution immediately for consideration - see below for
instructions - however, we can not guarantee its inclusion on the CD-ROM
at this point.)
In any case, please be prepared to submit your contribution before Aug
Contributions to the CD-ROM will not count as publications and all data
will be handled confidentially until the CD-ROM is released at the ASCB
Instructions for submission of data:
Please provide 1-5 image sequences. Indicate the desired playback rate
(frames/second), the original length of the recording and whether you
prefer normal or double display size in case the frame size is smaller
h240 pixel. Text should be included in the legend (see below) and not in
the movie itself. The movies will be looped, therefore it may be
advantageous to include a short fade in/out.
Format: UNCOMPRESSED QuickTime or AVI movie file (preferred), or numbered
file series (TIFF, BMP, PCX, or PICT, name format e.g. file0034.tif),
8-bit monochrome or 24-bit color.
Attention Mac users: PCs have a "darker" display gamma, so re-check your
movies after switching to linear or uncorrected gamma in the
Monitors(&Sound) control panel.
Size: maximum size w380 x h480 pixels
File Size: 50MB total (uncompressed)
Text: The explanatory text should be comprehensible by a non-specialist,
however, you don't need to introduce the concepts of fluorescence
microscopy, protein fusions or GFP itself since these topics will be
covered in the general introduction. There are no strict limitations
regarding length, but remember that the briefer, simpler and more concise
the explanations, the more likely that people will actually read it (and
watch your movies)!
- a brief, self-explaining legend for each sequence
- a background and discussion section, incl. a few (review) references
- a sketchy methods&materials section, also listing the equipment used.
Don't include vector construction procedures and the like.
You are welcome to provide a link to your home page if you have one.
Upon request send your data (on Mac- or PC-formatted CD-ROM, Iomega ZIP,
SyQuest SyJet, 1.2GB or 1.3GB MO disk) to
Dr. B. Ludin
Friedrich Miescher Institut
Dr. Beat Ludin, FMI, Maulbeerstr 66, 4058 Basel, Switzerland
Tel. +41 61 697 6697 / FAX +41 61 697 3976