> Would you tell me how many GFP gene (pEGFP-C1) do transfected cells need
> for us to detect fluorescence? (I used CMV promoter and HeLa as
> transfected cell : I don't mind any cell type :) )
> I think the transfected cells should need over 1 number in order to
> satisfy our dectection.
Indeed, cells are readily detectable with a single copy of CMV-driven EGFP.
> Thanks in advance..
You are very wellcome!
U09577 at uic.edu