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Sebastien PARIS nathalie.porchet at UNIV-ROUEN.FR
Fri Mar 13 03:19:26 EST 1998


Hi,

I have the same problem with the cell line I use: I have heterogeneous
fluorescence, even if I work with a monoclonal population. In this
population, the cells are stable for GFP and always in culture media with
G418. I think that is very difficult (may be impossible) to obtain a
population stable for GFP, that express every time a good and homogeneous
level of  GFP.
I think it's usual.

Sincerely,

Sebastien PARIS

 
___________________________________________
                  
INSERM U295
Faculte de Medecine-Pharmacie de Rouen  
Avenue de l'Universite
B.P. 97                                                        
76803 St-Etienne-Rouvray Cedex
France

Tel : (33)-02-35-65-52-74
Fax : (33)-02-35-66-12-78
E-Mail : nathalie.porchet at univ-rouen.fr
___________________________________________




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