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BFP stability

Beat Ludin ludin at FMI.CH
Sat May 16 06:57:50 EST 1998


Sharon Barr wrote:

>One of my coworkers is trying to localize a protein in cultured mammalian
>cells using BFP.  She finds that the blue photobleaches almost immediately.
>I do not see the same effect with GFP.  I was referring to this rapid
>photobleaching when I said instability.

Ok, so the answer is yes. BFP does bleach a lot faster than GFP 
(especially the "red-shifted" variety like GFP(S65T), EGFP, etc.). 

Beat



-----------------------------------------------------
Dr. Beat Ludin, FMI, Maulbeerstr 66, 4058 Basel, Switzerland
Tel. +41 61 697 6697 / FAX +41 61 697 3976




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