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Bacterial expression of GFPuv

Simon Foote foote at nrcbsa.bio.nrc.ca
Thu May 21 12:14:56 EST 1998


Has anyone expressed GFPuv in the periplasm of E. coli, either fused or
un-fused?  I'm attempting both using the OmpA leader sequence to direct
it to the periplasm.  So far, I haven't gotten any flourescing colonies
even though digests indicate the correct size fragments are present. 
I'm sequencing them to confirm this.  I suspect that it is possibly
going into inclusion bodies.

Thanks,
Simon
NRC-IBS
Novel Antibodies Group
Ottawa, Canada
simon.foote at nrc.ca



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