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PE vs FITC

Michal Opas m.opas at utoronto.ca
Fri May 29 10:34:40 EST 1998


Dear All,

A friend of mine experiences the following problem:
it appears as if samples double labelled with phycoerythrin 
and FITC "loose" FITC label.
When she does single labellings, they work fine.  However, 
in double labelled samples only the PE label is visible and 
the FITC label is gone.
I have no experience with PE whatsoever, so I am clueless.
Any suggestions?       

Thanks a lot
Michal

     
     Dr. Michal Opas
     Department of Anatomy & Cell Biology
     University of Toronto
     1 King's College Circle
     Medical Sciences Building
     Toronto, Ontario, M5S 1A8 Canada
     
       phone: (416) 978-8947
         fax: (416) 978-3954
      e-mail: m.opas at utoronto.ca
www homepage: http://www.utoronto.ca/anatomy/opas/start.htm 
   





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