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GFP mRNA expression

Mark Levasseur m.d.levasseur at newcastle.ac.uk
Wed Jul 7 06:05:13 EST 1999


Date: Wed, 07 Jul 1999 11:07:24 +0100
To: fluorpro at net.bio.net
From: Mark Levasseur <m.d.levasseur at newcastle.ac.uk>
Subject: GFP mRNA expression

Does anyone have any experience of obtaining expression from mRNAs coding
for dual GFP fusions of approximately 2 kilobases with extremely high GC
content? I am microinjecting into cells which normally express other GFP
fusion mRNAs very efficiently. Injection of these longer dual GFP mRNAs has
so far only resulted in expression in 1 of 24 cells injected. It seems that
the most likely explanation is problems with secondary structure formation
- does anyone know of a good way to avoid this?
Dr. Mark Levasseur
Dept. of Physiology
The Medical School
University of Newcastle upon Tyne
Framlington Place 
Newcastle  NE2 4HH

Tel  0191 222 6955
E-mail m.d.levasseur at ncl.ac.uk



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