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CFP/YFP FRET??

Jerry not_here at otherworld.net
Thu Jul 22 08:04:06 EST 1999


Is autofluorescence a possibility?

Jerry
Gordon at med.unc.edu

Andrei Popov wrote:

> Hello
>
> I tried it (same set of filters) in XLaevis cells and I saw
>
> that CYAN(+) cells that did not have YFP appeared "positive" in the FRET
> filter. I do not have a good explanation for this. In theory YFP should not
> get excited by 440 nm light...
>
> Regards
>
> Andrei
>
>    -**** Posted from RemarQ, http://www.remarq.com/?a ****-
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