I am looking urgently for a protocol allowing the simultaneous detection
of GFP and BrdU incorporation.
For BrdU incorporation studies, until now I used the kit form Boehringer
Mannheim (now Roche diagnostics).
The problem with this kit is, that cells are fixed in an ethanol-Glycine
buffer (pH 2), which destroys GFP fluorescence. Does anybody have an
idea, how to solve this problem?
PFA fixation and permeabilisation with 0.1% Triton does not work.
Josef P. Magyar, Ph.D.
Institut fuer Zellbiologie, HPM F27
Tel: + 441 1 633 33 54
Fax: + 441 1 633 10 69
Email: magyar at cell.biol.ethz.ch