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EGFP stables

Kathryn Sunn k.sunn at garvan.unsw.edu.au
Tue Jun 15 23:36:51 EST 1999


Has anyone ever heard of a cell line which stabley expresses a GFP fusion
protein, silencing the fluorescence of the GFP?? I have recently made a
stable cell line with the EGFP vector under the CMV promoter and it was
working really well, now after approximately 2 months in culture all the 36
lines of the fusion protein cell expressing cell line no longer fluoresce.
As you can imagine this is quite a lot of wasted time  and it would be
interesting to know if anyopne else has had the same problem, or knows if
this promoter is a bit unreliable?

Thanks

Kate

___________________________________________________________________
Kathryn Sunn  BSc (Hons)
Bone and Mineral Research Program
Garvan Institute of Medical Research
St Vincent's Hospital                          Tel:   (02) 295 8260
Sydney NSW 2010                                Fax:   (02) 295 8241
Australia                          email: k.sunn at garvan.unsw.edu.au
___________________________________________________________________






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