Has anyone ever heard of a cell line which stabley expresses a GFP fusion
protein, silencing the fluorescence of the GFP?? I have recently made a
stable cell line with the EGFP vector under the CMV promoter and it was
working really well, now after approximately 2 months in culture all the 36
lines of the fusion protein cell expressing cell line no longer fluoresce.
As you can imagine this is quite a lot of wasted time and it would be
interesting to know if anyopne else has had the same problem, or knows if
this promoter is a bit unreliable?
Kathryn Sunn BSc (Hons)
Bone and Mineral Research Program
Garvan Institute of Medical Research
St Vincent's Hospital Tel: (02) 295 8260
Sydney NSW 2010 Fax: (02) 295 8241
Australia email: k.sunn at garvan.unsw.edu.au