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Detection of EGFP in knock-in mice

Jing Yang jingyang at mail.med.upenn.edu
Thu Jun 17 10:57:51 EST 1999


I have trouble detecting EGFP expression in tissues of my knock-in
transgenic
mouse from cryosection (too much background) or immunohistochemistry
(clontech's polyclonal
antibody), though I did see predicted signal in platelets and spleenocytes
by FACS. 



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