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Detection of EGFP in knock-in mice

Jing Yang, by way of Mail Administrator jingyang at MAIL.MED.UPENN.EDU
Fri Jun 18 14:26:09 EST 1999


I have trouble detecting EGFP expression in tissues of my knock-in
transgenic
mouse from cryosection (too much background) or immunohistochemistry
(clontech's polyclonal
antibody), though I did see predicted signal in platelets and spleenocytes
by FACS.





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