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ELISA for EGFP antibodies

Jordi Barquinero, by way of Mail Administrator jbarquinero at iro.es
Tue Jun 22 02:08:32 EST 1999


We are trying to set up an ELISA assay to quantify antibodies against
EGFP. I would appreciate any suggestion on how much protein is needed to
coat the 96-well plates, and what is the optimal dilution of the
antibody? (we are using the Clontech anti-EGFP monoclonal Ab).

Thanks a lot

Jordi Barquinero
Institut de Recerca Oncologica
Hospitalet, Barcelona, Spain
jbarquinero at iro.es





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