I use Protease inhibitor cocktail tablets from Roche. They seem to work
well. Unfortunately they are very expensive. They inhibit a wider
spectrum of proteases than PMSF though. There are also more stable
analogs of PMSF available. Again they cost more than PMSF does.
Mike Moser, Ph.D. Tel: 206-543-6585
UW Department of Pathology FAX: 206-543-3967
Box 357705 moser at u.washington.edu
Seattle, WA 98195 http://faculty.washington.edu/moser
On Fri, 28 Jan 2000, simm wrote:
> Dear Protein experts,
>> I am currently running experiments requiring the use of PMSF. I have to
> prepare fresh
> solutions each time which as you may know takes up time and wastes a lot of
> reagent. Is there a way to prepare a solution which is stable in storage?
> are there other similar protease inhibitors? I am specifically interested in
> stability of PMSF in methanol, acetone and isopropanol over time. I have
> heard some colleagues store such solutions for up to a month, but I need
> confirmation from the scientific body.
>> Kindly, please respond to my email address:
>simm at worldnet.att.net>> Best regards,
>> Dr. Margaret Simm, PhD