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Green Fluorescent Protein Purification Short Course

gfp-1 meton at rci.rutgers.edu
Mon Jul 17 20:09:14 EST 2000

I have been asked to announce the following,

                  The State University of New Jersey
                        Campus at New Brunswick

             Center for Research and Education in Bioluminescence
                          and Biotechnology
                   Programs in Biotechnology, Presents:

Protein Purification: Isolation, Analysis, and Characterization of GFP,
A Five and One-Half Day Hands-On Laboratory Course Using the remarkable
Green-Fluorescent Protein (GFP), A Novel Marker For Gene Expression,
as the source material
                             July 23-28, 2000
                     and July 30-August 4, 2000

More than 600 scientists from around the world have strongly recommended
intensive course as an opportunity to develop protein research and
skills in a retreat setting.  Participants work hard, identify and solve
problems in the lab and enjoy camaraderie and good food and beer with

This five and one-half day laboratory course covers a wide variety of
conventional methods for protein isolation, purification, and
characterization.  The course format integrates hands-on laboratory
with classroom lectures, demonstrations, study breaks, and short

A special feature of the course is that all laboratory work will be
on the same starting sample (Aequorea GFP*), which will be purified from
exceedingly crude form to near homogeneity as judged by high performance
liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric
and capillary zone electrophoresis. This feature provides a continuity
purpose, integrating dozens of preparative and analytical protein
in a way that few competing courses can match.

A problem-solving approach will be used throughout the course.  Under
guidance of experienced lab instructors, participants will work in
of three to plan their own protocols, analyze data, and interpret
A student-teacher ratio not greater than 8:1 will be maintained and the
faculty coordinators will be present throughout the course.

*Note: The use of GFP from a recombinant source (E. coli) is also being
 as starting material due to its popularity within the scientific

For further details you can reach us,

by E-mail at:  meton at rci.rutgers.edu
by phone at:  (732) 932-9071 extension 225
by FAX at:  (732) 932-3633

for a brochure and further information please visit the GFP purification
short course official Web site at:

Notice: We will be discounting the registration fee by 20% for anyone
registering via the WEB!

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