I am trying to transfect COS-1 cells with a ds-red construct however am
having some difficulty being able to visualise the fluorescence of the
fusion protein. Does anyone have any suggestions? My cells were quite
confluent when I transfected them so I thought this may be the trouble, but
also our fluoro lamp is a little dodgy, so I was hoping they may look
brighter under the confocal.
Has anyone tried double labelling with EGFP, are they relative in brightness?
I let the cells transfect for approx 60 hrs so I thought this should be
plenty of time??
Any suggestions would be great!
Kathryn Sunn BSc (Hons)
Bone and Mineral Research Program
Garvan Institute of Medical Research
St Vincent's Hospital Tel: (02) 295 8260
Sydney NSW 2010 Fax: (02) 295 8241
Australia email: k.sunn at garvan.unsw.edu.au