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GFP tagged on both sides

softlad eemorrison at my-deja.com
Wed Oct 18 06:27:09 EST 2000


In article <Pine.GSO.4.21.0010052104170.16130-100000 at hgems>,
  "Andrej.Michalik" <amichal at uia.ua.ac.be> wrote:
> Dear netters,
> I wonder whether anyone ever produced GFP (or EGFP) fused to a protein at
> each end - "internal GFP", and whether it stayed fluorescent? Is it
> trial and error or should I better not try it?
> Thanx
> Andrej
>
> ------------------------------------------------------------------------------
>         A               andrej michalik
>        A A              department of biochemistry
>       A   A             university of antwerp (uia)
>      A     A            universiteitsplein 1
>     AAAAAAAAAAAAA       B-2610 antwerpen
>    A         AAA        belgium
>   A           A         tel: +32-(0)3-8202642
>                         amichal at uia.ac.be
> ------------------------------------------------------------------------------
>

Check out fig 1, JCB 148(3), 505-517 (2000). Also seem to recall people
using concatenated GFPs to increase signal with some success-GFP flanked
by GFPs. As always, try it and see.


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