> Hi GFPers,
> I've constructed a vector expressing GFP with a signal peptide of
secretion, in S. cerevisea.
> I would like to know if GFP, after extracellular secretion, could be
fluorescent and if yes, if it should be observed directly under microscope ?
The main problem in doing so would probably be dilution of the protein into
the growth medium. I would not expect that such 'fluorescent medium' would
be distinguishable from background in an conventional microscope.