We have constructed a receptor bearing GFP and did the necessary test to
see if things works
(CHO transient expression works like a charm, nice signal on the FACS).
So next thing was to have 3T3 cells express the GFP tagged receptor
stably (G418 selection),
unfortunately the FACS signal is not as high as in the CHO expression
and in some cases downright poor.
Might it be possible that the receptor quenches the GFP signal or are we
facing other problems?
Thanks for your time.
Ing. Walter Pouwels
Academic Medical Center
University of Amsterdam
Laboratory for Experimental Immunology
P.O. box 22700
1100 DE Amsterdam
Tel.: +31-20-566768756/ 68036/ 5662285