You are invited to attend a live, interactive, web-based instructional
"Quantitative Image and Data Acquisition for Fluorescent Specimens"
Advice from a Facility Director
Presented by Brian Matsumoto, Ph.D., University of California, Santa
Details are below. Connection lines are limited, so reserve yours
now. There is no charge to participate in this on-line seminar.
Thursday, 13-September, 1:00PM (Eastern time; 10 AM Pacific time.)
Duration: Approximately 1 hour.
Pre-register (required) at:
[If this link has wrapped, please re-build it in your web browser's
address bar. The line begins with "https" and ends with "RG=1" ]
Attendees will learn about issues affecting the quantitative accuracy
of fluorescence images acquired through a microscope and will pick up
tips and suggestions for improving image quality, making better use of
the camera's light collection abilities, and will learn the
nomenclature associated with digital imaging. Attendees will leave
with a better understanding of how to characterize and optimize their
optical system, camera, and software. Bring your questions to this
live, interactive web-based seminar.
- What is bit depth and when/why does it matter?
- What is dynamic range?
- Characterizing your camera's linear range.
- Noise and other sources of data uncertainty.
- Maximizing your camera's light collection capabilities.
- Setting the best exposures.
- Correcting for photobleaching.
- Collecting images in 3D.
About the presenter
Brian Matsumoto, Ph.D. is the Director of the Integrated Microscopy
Facility and is an Associate Adjunct Professor for the department of
Molecular, Cellular and Developmental Biology Department at the
University of California Santa Barbara. He is the author of Basic
Methods in Light Microscopy (Cambridge University
Press) and editor of Cell Biological Applications of Confocal
Microscopy (Academic Press).
Upcoming Webinar: "Deconvolving 3D Fluorescence Images for
Quantitative Analysis" (invitations will be sent separately).
Sponsored by Media Cybernetics (Image Pro and AutoQuant software) and
QImaging (precision CCD cameras)
This seminar requires that attendees use a Java-enabled browser with a
high bandwidth connection. Audio is via toll-free telephone.
There is no charge to participate in this on-line seminar.