The commercial product ,EPO, has a narrow band ( about 30 kD )on the
SDS-PAGE, but the purified EPO from my lab. has a broad band (22 - 30
kD)on the gel.
Does the commercial EPO contain homogeneous product by getting rid of
less-glycosylated product with gel filteration chromatography, or can the
homogeneous product be obtained from well-controlled culture condition?