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Recombinant glycoproteins expression in insect cell....

RONALD L SCHNAAR rschnaar at WELCHLINK.WELCH.JHU.EDU
Fri Sep 29 07:32:52 EST 1995


Dr. Chang

Your question is a very important one, which has been addressed by the
superb studies of Dr. Francis Castellino and colleagues at Notre Dame
University.  They report that twenty hours post-infection with
baculovirus, Sf9 cells produce recombinant glycoprotein bearing only
simple truncated N-linked structures with terminal mannoses.  However, as
time after infection progresses, the complexity of the N-linked glycans
increases, until at 60 hours post infection there are typical
tetra-antenary oligosaccharides with terminal sialic acids. 

An excellent review and example of these studies was published by F.J. 
Castellino, et al. in Methods of Enzymology 223, 168-185 (1993).  See 
especially the structures on pp. 180-181 and the Tables on pp. 182-184.

Being a bit removed from this area, it has been my impression that Dr. 
Castellino's findings have not been as widely appreciated as they might be
and your question is an excellent opportunity to discuss the work on the
net.  Can anyone else comment?

On 29 Sep 1995, [@e0GHq] wrote:

> Dear glyco-friends....
> 
> I heard that glycosylation in insect cell is poorly understood.
> 
> Especially in the case of recombinant glycoproteins expressed in 
> 
> insect cell, they say that the glycoproteins lack sialyltion and
> 
> has high-mannose structure instead of complex type that we can see
> 
> in mammalian glycoproteins. 
> 
> But in the mail above (sent by James Fethiere in NRC) describes that
> 
> his glycoproteins are 'highly' sialylated !!
> 
> Right now, I don't know how I should figure it out by myself !
> 
> Would you please give me some comments about this problems.And
> 
> I would be deeply appreciated if you suggest any reference on
> 
> this matter.
> 
> 
> 
> 
> 
> Thank you very much
> 
> 
> 
> ____________________________________________________________________
> --------------------------------------------------------------------
>  
> Kernee H. Chang
> 
> Lab. of Bio/Fermentation Tech.    E-mail: Kernee at bioneer.kaist.ac.kr
> Dept.of Biological Sciences       FAX: 042-869-2610
> Korea Advanced Institute of 
> Science and Technology(KAIST).
> 373-1, Koosung dong,
> Yoosung gu, Taejon, 
> Republic of Korea.
> 
> ____________________________________________________________________
> --------------------------------------------------------------------
> 
> 
> 





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