OK, glycochemists, here is a thought experiment:
Given a small amount of glycoprotein, heavily (and heterogeneously) O-
and N-glycosylated, and having been purified in the presence of
detergent (e.g. TX-100), what is the best way to get amino acid
composition and concentration ?
Is there anything wrong with traditional hydrolysis with 6N HCl followed
by HPLC detection of derivatized standards? What would be a suitable and
sensitive label? Will detergent interfere ? Will the oligosaccharides
refuse in some instances to come off? I don't think so, but maybe
someone has actual experience doing this.
roomen at hookup.net