Is anyone out there knows how I can obtain the ER alpha-glucosidase II, either
as a purified or recombinant form. I'd like to use it to confirm a high mannosestructure, Glc1Man9GlcNAc2, which was identified by MALDI-TOFMS. I was unable
to cleave the species by maltase (alpha-glucosidase, from S. ceravisae), which
cleaves terminal alpha-Glc linked to many other hexoses, but not mannose.
Thank you very much.
Ke-Wei Zhao, Ph.D.
Department of Biological Chemistry
UCLA School of Medicine, CHS 33-257
10833 Le Conte Avenue
Los Angeles, CA 90095-1737