>From irina Fri, 19 Jan 96 15:47:14 ARG remote from idehu
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>Date: Fri, 19 Jan 96 15:47:14 ARG
>From: Irina Mathov <irina at idehu.edu.ar>
>Message-ID: <672ao206 at idehu.edu.ar>
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>To: gycosci at net.bio.net>Subject: Succinyl wheat germ
>>Hi all
>I'm using biotinilated succinyl wheat germ to develope some western blots
>as Pierce indicates in the instructions but so far I have doubtfull results.
>When I use glycoproteins known to have terminal N Acetyl glucosamine the bands
>are very weak (I have tried different conc. of SWG)
>I couldn't find any references about the needs of cations in the solutions
>used for incubation (as for ConA).
>Does anyone know if SWG needs any cation to improve its binding?
>Any idea will be wellcome.
>Thanks a lot
>Irina Mathov
Irina Mathov
irina at idehu.edu.ar
