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Shantha Raju raju at aecom.yu.edu
Mon Jan 22 11:12:27 EST 1996

Irina Mathov wrote as:
>Hi all
>I'm using biotinilated succinyl wheat germ to develope some western blots
>as Pierce indicates in the instructions but so far I have doubtfull results.
>When I use glycoproteins known to have terminal N Acetyl glucosamine the bands
>are very weak (I have tried different conc. of SWG)
>I couldn't find any references about the needs of cations in the solutions
>used for incubation (as for ConA).
>Does anyone know if SWG needs any cation to improve its binding?
>Any idea will be wellcome.
>Thanks a lot
>Irina Mathov

                                        Irina Mathov
                                        irina at idehu.edu.ar

I believe WGA also needs cations like Ca2+ and Mg2+. Atleast for affinity
chromatography I normally use PBS with Ca2+, Mg2+ and azide. Also may be
you need to try just biotinylated WGA instead of S-WGA.

I hope this helps.

Shantha Raju

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