IUBio GIL .. BIOSCI/Bionet News .. Biosequences .. Software .. FTP

O-linked sugars

Sun Sep 22 20:12:29 EST 1996

We have an antibody (MUD50) that recognises Ser/Thr-O-GlcNAc (beta comformation) in 
the context of a PTVT repeat.  It will recognise a single T-GlcNAc and it also 
recognises S-GlcNAc, but I am unsure whether the there are other amino acid sequence 
constraints on the antibody recognition, eg it may require adjacent proline residues.   
Thus, the lack of a reaction with MUD50 might be due to the particular amino acid 
sequence.  We would be eager to find out.

Cheers, Martin Slade


>To:            glycosci at net.bio.net
>Subject:       O-linked sugars
>From:          (k.c.breen at dundee.ac.uk)
>Date:          19 Sep 1996 14:38:32 GMT
>I would like to check for the presence of Ser/Thr-O-GlcNAc on a protein.
>Looking through the appropiate catalogues, I cannot find a lectin that
>specifically labels this sugar group. I would be grateful if somebody could
>give me details of how I may assay for this particular sugar linkage.
>Kieran Breen, Dept. of Pharmacology,                  
>University of Dundee,                   
>Ninewells Hospital & Medical School,    
>Dundee DD1 9SY,Scotland, U.K.                                              
>k.c.breen at dundee.ac.uk                  
Martin Slade,
School of Biological Sciences,
Macquarie University,
NSW 2109,
FAX  (61 2) 850 8174
Phone(61 2) 850 8210

More information about the Glycosci mailing list

Send comments to us at archive@iubioarchive.bio.net