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known ConA binding proteins

Corne Stroop cjms at boc.chem.ruu.nl
Tue Sep 24 11:27:31 EST 1996

Craig Webster wrote:
> In article <jdb-230996164547 at wintermute.bioc.rice.edu>, John Bishop
> I use con A to bind glycoforms of alpha-1-acid glycoprotein (orosomucoid) in
> my work. As far as I know con A will bind to any protein that has bi-
> antennary glycans attached as this is the glycan motif that con A recognises.

Well, 'binding' should be replaced by 'retarded'. As David Ashford
already pointed out: ConA binds to oligomannose and hybrid type glycans.
So, using ConA can result in three (!) pools. A unretarded pool of
proteins bearing no or only tri/tetra-antennery glycans, a retarded pool
of glycoproteins bearing bi-antennary glycans, and a bound pool of
glycoproteins with oligomannose or hybrid type structures.

Yours Sincerely,


 Corné JM Stroop
 Department of Bio-Organic Chemistry
 Bijvoet Center for Biomolecular Research
 Padualaan 8
 NL-3584 CH  Utrecht, Netherlands
    PHONE: 31-30-2533752    FAX: 31-30-2540980
        E-MAIL: cjms at ruucj1.chem.ruu.nl

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