For Sunny's question of unprotection of glucuronide:
Dissolve your compound in methanol (cold), flux ammonia for 1hr during stirring,
and store in a refrigerator o/n. Acetate is recovered by filtration. See
J. Histochem. Cytochem. 2: 209-229, 1954 for details on similar compound.
Ke-Wei Zhao, Ph.D.
Dept. Biol. Chem.
UCLA School of Medicine
Los Angeles, CA 90095