We localized lectin binding sites on Xenopus laevis eggs. At the
electron microscope such oligosacharides are asociated to the
glycocalix. We just started biochemical experiments to study such
glycoproteins by SDS-PAGE and Western-blotting with biotinylated lectins
and streptavidin-peroxidase. The data suggest that some lectin reactive
glycoproteins are lost during the sample preparation while others are
retained. The preparation is suited for retaining plasma membrane
proteins. By analizing pellets or supernatants which are intermediates
in our purification method, we identified some protein bands reactive to
the particular lectin we are interested.
We're looking for a sample preparation suited for retaining both plasma
membrane and the associated extracellular matrix or the latter only.