Accompanying request, received at the Maize Genetics COOP, forwarded to the
maize net.
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Dear sir/Mme:
I am a graduate student at the dept of Biology, Mcgill University,
Montreal, Canada. My Ph.D. project involves some molecular studies on
maize and some of it wild relatives (teosinte). The reason I am writing
you is that I'm in need for some advice. I have been wanting to generate
a large number of maize (W22) seeds for RNA extraction from etiolated
seedlings but unfortunately I have come through a problem; My W22 plants
grown in our greenhouse conditions develop normally and flower normally
but do NOT shed any pollen. The same problem was encountered by another
lab colleage. In this way, I have wasted a lot of time trying to get the
seed I need for my experiments.
I would really appreciate it if someone can answer me back with an idea
of why is this happenning and what should I do to avoid it. Also I will
really appreciate it if someone can help me by providing me with some
W22 seed (300 to 400 seeds) to be able to start my experiment until the
problem is solved.
Thank you very much
Nabil Elrouby
Dept of Biology
McGill University
1205 Dr. Penfield Ave
Montreal, PQ
H3A 1B1
Canada
tel 514-398-2141
fax 514-398-5069
e.mail: nelrou at po-box.mcgill.ca