Pat,
We use the Dellaporta microprep method from the Maize Handbook to extract
DNA from a single seedling leaf. Addition of RNase to the prep improves
PCR results.
-Martha
>We need to isolate DNA (for PCR) from many thousands of maize plants.
>We've found some very quick/easy extraction protocols that work on fresh
>tissues. However, the big stumbling block is tissue grinding. We would
>like to avoid the necessity of freeze-drying the tissue prior to
>extraction, but so far we haven't had good luck grinding fresh tissue in a
>paint shaker.
>>Has anyone worked out a reliable, easy protocol from fresh tissue?
>>Pat
>>>>Patrick S. Schnable
>Professor of Plant Genetics
>G405 Agronomy Hall
>Iowa State University
>Ames, IA 50011 USA
>schnable at iastate.edu>515-294-0975 (office)
>515-294-2299 (fax)
>http://www.public.iastate.edu/~schnable/
===================================================================
Dr. Martha G. James
Adjunct Assistant Professor
Department of Biochemistry, Biophysics, and Molecular Biology
2152 Molecular Biology Building
Iowa State University, Ames, IA 50011
Phone: (515) 294-3818
FAX: (515) 294-0453
e-mail: mgjames at iastate.edu
