Pat,
While on postdoc in Jim Peacock's lab I tried out a "sap-extractor" built
by the CSIRO engineers. It had motor-driven metal rollers through which a
single fresh seedling leaf was passed. The sap ran down the rollers for
collection in a tube, and it had an automatic wash fluid dispenser to clean
the rollers between samples. It was designed for virus sampling. It was
fast and easy, so we tried it for genomic DNA preps. The DNA was usually
too degraded for Southerns, but I'll bet it would work for PCR.
Regards, Tom
At 08:11 AM 11/25/98 -0600, you wrote:
>We need to isolate DNA (for PCR) from many thousands of maize plants.
>We've found some very quick/easy extraction protocols that work on fresh
>tissues. However, the big stumbling block is tissue grinding. We would
>like to avoid the necessity of freeze-drying the tissue prior to
>extraction, but so far we haven't had good luck grinding fresh tissue in a
>paint shaker.
>>Has anyone worked out a reliable, easy protocol from fresh tissue?
>>Pat
>>>>Patrick S. Schnable
>Professor of Plant Genetics
>G405 Agronomy Hall
>Iowa State University
>Ames, IA 50011 USA
>schnable at iastate.edu>515-294-0975 (office)
>515-294-2299 (fax)
>http://www.public.iastate.edu/~schnable/>
