There was an alkali based protocol for rapid treatment of tissue for PCR
published some time ago (Klimyuk et al, 1993 Plant Journal 3:493). I tried
the technique on a variety of maize tissues. Results with leaves were
quite variable, but embryo and dry endosperm pieces worked quite well. As I
recall, one challenge of the protocol was to make sure the alkali was
properly neutralized before PCR.
Best wishes,
Randall Kerstetter
>Hi,
>I would like to genotype my mutator-gene knock out seeds directly by PCR
>analysis using DNA extracted from maize endosperm. Has anybody tried this
>or developed a protocol for extracting DNA from dry endosperm?
>>Any help would be appreciated. thanks,
>Amie
>>--
>Amie E. Franklin
>****************
>franklin at candelab.berkeley.edu>345 LSA, MCB Dept.
>U.C.Berkeley
>Berkeley, CA 94720
>lab (510) 643-8277
>fax (510) 643-6791
___________________________________________________________
Randall Kerstetter, PhD randallk at mail.sas.upenn.edu
University of Pennsylvania Dr. Scott Poethig's Lab
Plant Sciences Institute voice: (215) 898-8916
Department of Biology, 304 Mudd fax: (215) 898-8780
Philadelphia, PA 19104-6018
