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DNA from endosperm

Randall Kerstetter randallk at sas.upenn.edu
Tue Apr 13 09:11:44 EST 1999

There was an alkali based protocol for rapid treatment of tissue for PCR
published some time ago (Klimyuk et al, 1993 Plant Journal 3:493).  I tried
the technique on a variety of maize tissues.  Results with leaves were
quite variable, but embryo and dry endosperm pieces worked quite well. As I
recall, one challenge of the protocol was to make sure the alkali was
properly neutralized before PCR.

Best wishes,

Randall Kerstetter

>I would like to genotype my mutator-gene knock out seeds directly by PCR
>analysis using DNA extracted from maize endosperm. Has anybody tried this
>or developed a protocol for extracting DNA from dry endosperm?
>Any help would be appreciated. thanks,
>Amie E. Franklin
>franklin at candelab.berkeley.edu
>345 LSA, MCB Dept.
>Berkeley, CA 94720
>lab (510) 643-8277
>fax (510) 643-6791

Randall Kerstetter, PhD         randallk at mail.sas.upenn.edu
University of Pennsylvania          Dr. Scott Poethig's Lab
Plant Sciences Institute              voice: (215) 898-8916
Department of Biology, 304 Mudd         fax: (215) 898-8780
Philadelphia, PA 19104-6018

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