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Postdoc available: Nuclear-Cytoplasmic Interactions in Maize

Roger P. Wise rpwise at iastate.edu
Mon Aug 16 11:33:07 EST 1999

Molecular Genetics of Nuclear-Cytoplasmic Interactions in Maize

Postdoctoral Research Associate available August 1.  The candidate 
will be responsible for the isolation and functional characterization 
of nuclear (Rf)  genes that mediate mitochondrial RNA processing and 
the restoration of male fertility in T-cytoplasm maize.  Male 
sterility in T-cytoplasm maize is due to the presence of a novel 
mitochondrial gene, T-urf13.  Full (or partial) fertility restoration 
of T-cytoplasm is mediated by one of three (Rf1, Rf8, or Rf*) nuclear 
restorers, in combination with the Rf2 restorer.  Rf2 encodes a 
protein highly similar to mitochondrial aldehyde dehydrogenases; 
Rf1-, Rf8-,and Rf* each mediate discrete T-urf13 transcript 
processing events.  This USDA/NRI-funded project is to physically 
characterize the genomic region spanning Rf1, use AFLP-transposon 
display to clone and characterize additional mutant alleles of Rf1, 
and to test the functionality of candidate Rf1 sequences via  our 
newly developed T-cytoplasm, transformation system.  The candidate 
will have access to a wide array of rf1-m mutants and recombinant 
populations as well as BAC library resources and high-throughput 
sequencing capability.

Literature references:

Wise, RP, CL Dill, and PS Schnable. 1996.  Mutator-induced mutations 
of the rf1 nuclear fertility restorer of T-cytoplasm maize alter the 
accumulation of T-urf13 mitochondrial transcripts. Genetics 

Dill, CL, RP Wise, and PS Schnable. 1997.  Rf8 and Rf* mediate unique 
T-urf13-transcript accumulation, revealing a conserved motif 
associated with RNA processing and restoration of pollen fertility in 
T-cytoplasm maize. Genetics 147:1367-1379.

Wise, RP, CR Bronson, PS Schnable, and HT Horner. 1998. The genetics, 
pathology, and molecular biology of T-cytoplasm male sterility in 
maize.  Advances in Agronomy 65: 79-130.


Ph.D. in genetics, molecular biology, plant physiology, or related 
field.  Experience in manipulation and cloning of large DNA 
fragments, gene mapping, computational analysis, and/or cereal 
transformation is desirable.  Initial appointment is for two years; 
salary starts at $33,026 per year + benefits.  Some limitations on 
citizenship may apply (see below).  Highly motivated individuals 
should send curriculum vitae, reprints, and references (name, e-mail, 
address, phone, and fax no.) to:

Dr. Roger Wise, USDA-ARS
Department of Plant Pathology
351 Bessey Hall
Iowa State University
Ames, IA, 50011
Telephone:   (515) 294-9756
Fax: 	    (515) 294-9420
E-mail:	    rpwise at iastate.edu

About Iowa State University

Ames (http://www.ames.ia.us/) is a small city of 50,000 people, about 
half of who are students 
m).  Affordable housing is available as well as a first-rate public 
transportation system.  It is also easy to get around town by 
bicycle.  The city and the University  (http://www.iastate.edu/) have 
numerous parks and recreation facilities, and the Iowa State Center, 
home of Hilton Coliseum, C.Y. Stephens Auditorium and Fisher Theater, 
hosts numerous music concerts, live performances, and athletic events 
throughout the year.

[Citizenship limitations on this position (funded through a 
NRI-competitive grant to USDA-ARS), requires that it be filled by 1) 
citizens of the United States, 2) a citizen of a country which is a 
member of a defense treaty, eg., NATO, SEATO, RIO treaty or 3) by 
citizens of countries which the U.S. congress has specifically 
exempted from the restricting legislation.  These countries include: 
Argentina, Australia, Bahamas, Belgium, Bolivia, Brazil, Canada, 
Chile, Columbia, Costa Rica, Cuba, Denmark, Dominican Republic, 
Ecuador, El Salvador, France, Germany, Greece, Guatemala, Haiti, 
Honduras, Iceland, Italy, Japan, South Korea, Luxembourg, Mexico, 
Netherlands, New Zealand, Nicaragua, Norway, Panama, Paraguay, Peru, 
Philippines, Portugal, Spain, Thailand, Tobago, Trinidad, Turkey, 
United Kingdom, United States, Uruguay, Venezuela, Ireland, Israel, 
(South Vietnamese, Cambodian, and Laotian refuges paroled into the 
U.S. after January 1, 1975), (Aliens from Cuba, Poland, South 
Vietnam, countries of the former Soviet Union, Estonia, Latvia, and 
Lithuania admitted to the U.S. for permanent residence), (Natives of 
Guam, Puerto Rico, and the Northern Mariana Islands), and (Nationals 
of the Peoples Republic of China that were in the U.S. on or before 
June 5, 1989 up until April 11, 1990, and qualify under the Chinese 
student protection act of 1992)]
Roger Wise, USDA-ARS
Department of Plant Pathology
409 Bessey Hall
Iowa State University
Ames, IA
50011-1020  USA
Phone:  515-294-9756
Fax:    515-294-9420
E-mail: rpwise at iastate.edu

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