Ed,
Comprehensive arrayed cDNA libraries, implies comprehensive cDNA resources,
maybe a cart-before-the-horse issue.
1, 2, 3, 4, 10, 14, 16 all need done, priority should be set by which
technologies are ready and cost-effective, not wishful thinking.
I don't see the point of #15 (one or more very large populations), IBM will
give the resolution of 900 individuals at the operating cost of 300. If you
are going to fine-map it is in a population segregating for your trait of
interest.
Is #13 (SSRs) more about marketing and distribution then about development?
MIke
Ed Coe wrote:
> To Maize Netters:
> Before, during, and after the 1999 Maize Genetics Meeting in
> Wisconsin, and at the Maize Forum 2000 in San Diego, an ad
> hoc Maize Committee has requested and received information
> about resource deficiencies that are limiting the
> productivity of maize genetics research in the public
> sector. Our results indicate the following major needs, in
> these approximate priority orders:
>> 1. Improved maize transformation capacity in the public
> sector [for your information, an action plan is being
> undertaken to bear this need to the Interagency Working
> Group for Plant Genomics].
>> 2. Better expression profiling tools.
>> 3. Comprehensive arrayed cDNA libraries.
>> 4. Tissue specific and developmentally controllable
> promoters.
>> 5. More community building activities, particularly for
> attracting new scientists to maize genetics.
>> 6. Less emphasis on megagrants, more on individual
> investigator awards.
>> 7. Improvements in the user-friendliness of MaizeDB.
>> 8. Better coordination of tool development for the community.
>> 9. Improved comparative data and bioinformatics, for the
> grasses and between maize mapping populations.
>> 10. Fully accessible, appropriately redundant enhancer
> traps, promoter traps, and knockouts systems in the public
> sector for maize reverse genetics.
>> 11. Improved reverse genetics tools in other grass species
> (e.g., rice) to allow comparisons to orthologous maize mutants.
>> 12. More complete and more accessible storage and
> distribution of maize EST data and clones.
>> 13. More maize SSRs available inexpensively to all.
>> 14. Large mutagenized (small deletions, EMS, transposon
> tagged) populations, in an inbred (B73) background.
>> 15. One or more very large (>2000 progeny) populations
> mapped with all of the available RFLP, SSR, etc. markers
> that can then be used for fine structure mapping by all
> maize researchers.
>> 16. Proteome knowledge and research, expression,
> polymorphism, and screening.
>> 17. Tools for comparing across different maize maps for QTL
> work involving different markers for different maps, and
> across species.
>> 18. Bulk purchasing of reagents needed by the maize genetics community.
>> How would you rate the priority of these needs, and any
> others that you may wish to add? Please reply to the maize
> net, maize at net.bio.net, where bulletin-board communications
> are appropriate.
>> Do you have any suggestions as to solutions for these
> limitations?
>> At future Maize Genetics Conferences, floor discussions will
> be carried out in greater depth, particularly as to how we
> might bring about the improvements that we all feel are
> needed. Thank you for your time and assistance.
>> Sincerely,
> Ed Coe and Jeff Bennetzen for the ad hoc Maize Committee.
> ---
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