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Microarray announcement

Vicki L. Chandler chandler at Ag.Arizona.Edu
Wed Aug 23 21:50:33 EST 2000

Maize Microarrays for Endosperm and Immature Ear Libraries Now Available

The microarray team at the University of Arizona has completed the 
arraying of two EST collections sequenced by the Maize Gene Discovery 
Project.  These arrays are now available for purchase through the 
ZmDB web site.  The price is $150 for two slides.  Below, a brief 
description of each array is provided.  For more information on the 
arrays and for ordering, please visit the web site: 
http://www.zmdb.iastate.edu/zmdb/microarray  to stay informed about 
the release of subsequent microarrays consult the progress pages 
linked to this site.  Please note that while this web site is active, 
it is still under development.  Currently the web site contains 8 
pages (overview, progress, controls, libraries, arrays, protocols for 
hybridization, ordering and links). Data from experiments with the 
arrays performed by the Maize Gene Discovery project staff will be 
posted soon.   Control experiments indicate that the arrays provide 
highly reproducible hybridization results.

Endosperm Array: Arrays contains 8,064 ESTs from the 605 library and 
93 controls.  Each EST is printed 2X and each control is printed 4X 
to produce 16,500 spots.   The arrays were printed in two formats: 
100 slides contain duplicate arrays; 200 slides contain a single 
large array with duplicate spots.

Immature Ear Array: Arrays contain 4,980 ESTs from the 606 library 
and 111 controls.  Each EST is printed 3X and each control is printed 
6X to produce 15,606 spots.  All slides were printed with a large 
single array with replicate spots.

We are supplying two slides because we highly recommend that you 
perform two comparisons with each pair of RNAs, i.e. label RNA "A" 
with cy5 and RNA "B" with cy3 dye for hybridization to the first 
slide and then reverse the labeling on your second slide.

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