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Hybridization with rDNA

MARCEL QUINT Quint at 350-ipsp.ipsp.Uni-Hohenheim.DE
Thu Nov 16 04:16:56 EST 2000

Dear colleagues,

I want to check plasmid inserts in a Southern HYB for clones with 
repetitive sequences. So I am planning to hybridyze with maize 
genomic DNA to identify these rDNA containing clones. I am not sure 
about the amount of labelled probe to use for this HYB. For a normal 
Southern with a certain fragment as probe i use 50-100ng 
(P32-labelling). But is this enough to cover all species of rDNA 
within genomic DNA?


Marcel Quint

Universitat Hohenheim
Institut fur Pflanzenzuchtung, Saatgutkunde und Populationsgenetik
Fruwirthstrasse 21
70593 Stuttgart
Tel# 0711/459-2697 o. -3491
Fax# 0711/459-2343


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