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Postdoctoral Research Associate, UC Berkeley

Barbara Alonso balonso at nature.berkeley.edu
Tue Apr 10 13:40:08 EST 2001

Postdoctoral Research Associate
Department of Plant and Microbial Biology
University of California, Berkeley, CA USA

A postdoctoral position is available immediately to participate in a 
project focused on the generation and characterization of Ds-mediated 
gene delivery in wheat. Recently it was shown that such a system 
leads to a dramatic increase in gene expression stability in barley 
(Koprek, T., et al., Transposon-mediated single-copy gene delivery 
leads to increased transgene expression stability in barley. Plant 
Physiology, 2001, accepted). In the present study, wheat plants 
containing transgenes delivered with the Ds-system will be 
characterized in terms of transgene expression stability relative to 
barley and to genes introduced by Agrobacterium and microparticle 
bombardment.  Characterization of the physical nature of gene 
insertion sites e.g., sequence, redundancy, methylation state and 
chromatin condensation will be carried out, and comparisons of the 
differences and similarities between the two plant species will be 
done. Candidate will also be involved in studies focused on assessing 
the impact of in vitro culturing and other stresses (e.g., nutrient, 
water) on transgene expression stability of Ds-delivered transgenes 
compared to those delivered by other methods. The successful 
candidate will have monocot transformation experience and advanced 
skills relating to the biochemical and molecular analysis of 
transgenic plants and the creation and analysis of vectors.  This 
project is expected to last a minimum of two years with a possibility 
of extension.  Please send curriculum vitae (preferably by e-mail) 
and the names, phone numbers and e-mail addresses of three references 
to Dr. Peggy G. Lemaux, 111 Koshland Hall, University of California, 
Berkeley, CA 94720; FAX:  510-642-7356; e-mail: 
balonso at nature.berkeley.edu.  The University of California is an 
equal opportunity/affirmative action employer.

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