The Clontech-Kit for SMART-cDNA-Synthesis is the best I know. The
Fast-Track I don't know - I think the best one is the QIAGEN
RNeasy-Kit.
Good luck!
Thomas
At 2:33 PM +0000 11/29/01, Veronique Fontaine wrote:
>Good morning,
>>I planned to build a cDNA library from 5 day's old maize tissue and
>tought to use
>>FastTrack 2.0 kit from Invitrogen
>for mRNA isolation directly from the tissue
>and
>SMART cDNA library construction kit from Clontech
>to built the library
>>What do you think of these 2 kits for maize? have you got any other
>good kit references for maize? Is it difficult to obtain mRNA from
>maize? Do you know the best method to do it?
>>Thank you for your help
>>Veronique Fontaine
>>>School of Biochemistry and Molecular Biology
>University of Leeds
>Leeds LS2 9JT, UK
>Phone 44 113 233 1435
>Fax 44 113 233 3167
>email bmbvf at bmb.leeds.ac.uk
