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maize cDNA library

tbutter at gwdg.de tbutter at gwdg.de
Tue Dec 4 11:24:44 EST 2001

The Clontech-Kit for SMART-cDNA-Synthesis is the best I know. The 
Fast-Track I don't know - I think the best one is the QIAGEN 

Good luck!

At 2:33 PM +0000 11/29/01, Veronique Fontaine wrote:
>Good morning,
>I planned to build a cDNA library from 5 day's old maize tissue and
>tought to use
>FastTrack 2.0 kit from Invitrogen
>for mRNA isolation directly from the tissue
>SMART cDNA library construction kit from Clontech
>to built the library
>What do you think of these 2 kits for maize? have you got any other
>good kit references for maize? Is it difficult to obtain mRNA from
>maize? Do you know the best method to do it?
>Thank you for your help
>Veronique Fontaine
>School of Biochemistry and Molecular Biology
>University of Leeds
>Leeds LS2 9JT,  UK
>Phone 44 113 233 1435
>Fax   44 113 233 3167
>email bmbvf at bmb.leeds.ac.uk

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