[Medicago] chloroplast-free DNA

[medicago from net.bio.net]

Hi

 

I'm trying to isolate a lot of CP-free DNA for sequencing without going
to the trouble of nuclear isolation.

I've germinated seed in the dark and collected hypocotyls (about 6g).
However, based upon a similar approach with Brassica, I don't think I'll
get enough DNA. I suspect that compared with leaves, the hypocotyls have
big cells and thus fewer nuclei per gram

 

Any suggestions to get increased CP-free biomass?

 

Thanks

 

Chris

 

___________________________

Chris Town

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