Hello netters!
I have some questions, concerning double stranded DNA.
Does somebody know where can I find or how to calculate:
1. The maximum bond angle or angle of rotation between neighbouring base
pairs.
Solvent - water and/or TAE 0,004 M, pH=8,0
2. The forces (energy) or funktion of: altering the angle of rotation
from 5 to 0
degree
3. Energy to transform a random coiled DNA to a helical DNA with lowest
potential energy
(relation of radius of coil to angle of rotation for a given contour
length). Segmental transformation is also possible.
4. The explicit value for the bending rigidity constant and likewise for
the torsional rigidity constant.
I would like to get these data formulated not in related quantities. It
would be also helpful to get it with neglect of the anisotropy of the
double helix.
Thank You for giving hints to solve these problems.
Dr. Manfred Peska,
Institute of Medical Physiology
University of Vienna
E-mail: yuri.koshelnick at univie.ac.at
