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how to generate homo-dimer structure from monomer

Angel Gutierrez angel at inoc.chalmers.se
Mon Jun 23 07:44:24 EST 1997


Chyh-chong Chuang wrote:
 
>  "TO FORM THE BIOLOGICALLY ACTIVE DIMERIC MOLECULE, THE SECOND MONOMER NEEDS
>  TO BE GENERATED BY USING THE FOLLOWING MATRIX.
> 
>  SYMMETRY1   1 -0.499940  0.866060 -0.000030       -0.00159
>  SYMMETRY2   1  0.866060  0.499940 -0.000030        0.00002
>  SYMMETRY3   1 -0.000010 -0.000040 -1.000000        0.00153
> 
>   How to generate another monomer from this information? Or I need other
>  information to do this?
> 
>   Your kind response will be greatly appreciated.
>   Thank you very much.
> 
>  ccchuang
> 
>   ============================================
>     Chyh-Chong Chuang
>     Institude of Biological Chemistry,
>     Academia Sinica,Taipei, Taiwan
>   --------------------------------------------
>     Phone: 886-2-7858981 ext 7091
>     Fax: 886-2-7883473
>     E-Mail: ccchuang at gate.sinica.edu.tw
>   ============================================

	Yu need to transformate trh coordinates this way:

		Y=A*X+B

where
	X vector with the three coordinates of model
	Y vector with the three new coordinates
	A 3x3 rotation matrix
	B vector of translation

	You van make a program or programs like X-plor or packages like
CCP4 can do it for you.

	Hope it helps

	Angel
-- 
*======================================================================*
      Angel Gutierrez Rodriguez                 CURRENT ADDRESS    
     agr at sauron.quimica.uniovi.es            angel at inoc.chalmers.se 
            X-ray Group                         Oorganisk kemi  
 Dpto. de Quimica Fisica y Analitica       Chalmers Tekniska Hogskola 
       Universidad de Oviedo                       Goteborg 
              SPAIN                                 SWEDEN    
*======================================================================*
Once's happenstance,twice's coincidence,the third time is enemy action.
  Play, Mr. Bond. You'll need a great deal of luck to get out of this.
      I'm fit, I'm calm, I'm smooth, and I'm always on the limit.
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