Iain Johnstone asks:
>Does anyone have a method for adding a single T to the OH end of a blunt
>cut plasmid to permit cloning of Tac amplified PCR products directly. I
>assume it is possible using a dideoxy T and terminal transferase,
>however an actual method that is known to work would be useful.
Just such a method was published in Nucleic Acids Research (vol 19 (5),
p 1156) in 1991 by Holton and Graham. I have not tried it myself however.
Graham Clark
