I am an undergraduate student who has an interest in trichomonad
flagellates. I would like to stain the nucleus with a fluorescent dye
but have had no luck. DAPI and propidium iodide give no reaction
although the positive control (Tetrahymena) stains beautifully. I would
appreciate any information on procedures for fluorescently labelling
trichomonad nuclei for observation with microscopy, especially confocal
microscopy. Also, does anyone know why the "augusta" group can be
cultured in vitro and the "muris" group can not? Thank you for your help.
Nathaniel Hawkins
hawkina at mail.auburn.edu
