Dear Damiana. Human infection with T. trichura is common in Libya and has been recorded at Tripoli. I would suggest you send your request to :
Professor Mabrouk K. Zaid
Head, Department of Medical Parasitology
Faculty of Medicine, Al-Fatah Medical University.\
Tripoli, Libya
as well as to:
Director,
School of Tropical Medicine,
Kolkata,India.
Also try contacting Dian R. Bell, He has an access to Hospital of Tropical Diseases,London and himself a nematode man (Strongyloides stercoralis) and would surely help you. Human gut paraites (alive) can be obtained through purgation unless surgical probing. Had you take Enterobius vermicularis, the material could have been obtained abundantly. Best wishes for your continued progress with a very interesting project. H. Farooqi.
--- On Tue, 16/12/08, parasite-request from oat.bio.indiana.edu <parasite-request from oat.bio.indiana.edu> wrote:
From: parasite-request from oat.bio.indiana.edu <parasite-request from oat.bio.indiana.edu>
Subject: Parasite Digest, Vol 39, Issue 1
To: parasite from magpie.bio.indiana.edu
Date: Tuesday, 16 December, 2008, 10:34 PM
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Today's Topics:
1. Urgently looking for specimens of human whipworm (Trichuris
trichiura) (Damiana Ravasi)
----------------------------------------------------------------------
Message: 1
Date: Mon, 15 Dec 2008 11:23:14 +0200
From: "Damiana Ravasi" <damianaravasi from gmail.com>
Subject: [Parasitology] Urgently looking for specimens of human
whipworm (Trichuris trichiura)
To: parasite from magpie.bio.indiana.edu
Message-ID:
<81f2193c0812150123p153855c7wc53fa0f2771b5646 from mail.gmail.com>
Content-Type: text/plain; charset="iso-8859-1"
Dear all,
My name is Damiana Ravasi, I am a PhD candidate collaborating with Dr
Nichola Illing (Dept of Molecular Biology at UCT) on a project to
investigate whether baboons on the Cape Peninsula share the same parasites
as humans.
We have successfully amplified the ITS1 and ITS2 regions from genomic DNA
from one Trichuris worm isolated from a baboon gut, using the NC2 and NC5
primers (Cutillas et al 2002 Parasitol Res 88: 574-582)...but have not been
able to identify the corresponding sequence for Trichuris trichiura from
Genbank. We have tried to obtain a human Trichuris trichiura locally, but
without success. Next best would be to try and get the sequence of Trichuris
trichuira from humans from another source.
*Has anyone ever managed to sequence the human Trichuris trichiura ITS1 and
ITS2?
Or would anyone have an aliquot of Trichuris trichiura genomic DNA or the
nematode specimens which you would willing to send to us?*
Thank you very much
Damiana Ravasi
--
Damiana Ravasi
UCT Baboon Research Unit (BRU)
Zoology Department
University of Cape Town
Rondebosch 7701
South Africa
Tel: +27 (0)21 6503645
Fax: +27 (0)21 6503295
Cell: +27 (0)72 1908305
E-mail: damianaravasi from gmail.comhttp://www.baboonsonline.com/bru/project/parasites
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